Hi Danni,
First of all, you are crazy talented! Wow. The stain glass mosaics are incredible.
I was intrigued by your question to open a second store, because I have the same question for myself and my store. Just by looking around Etsy, it appears that shops with a single theme (look and feel) are the most popular.
About your shop and your question:
It is clear that you sell different items, but your shop doesn't look disjointed at all. If I were you, one of the main things I would consider is that you have 29 items in your shop. If you create a new store, you will have about 15 items in each shop - that's not very many items.
Given how much work it is to set up one shop, consider the amount of time it will take to manage two shops and keep those shops stocked with your beautiful items.
For right now, I would stick with one shop. However, if you do decide to create a second shop, I agree with Emma's idea of dividing the items.
Good luck!